Vinca Lab180

MethSpec: a simple and efficient tool for evaluation of MSP primer specificity

MethSpec online tool

Note: The primers should be entered in 5'-3' orientation.
Primer concentration   mol/l
Cation concentration   mol/l
Annealing temperature   °C
Forward primer (max 35bp) 
Reverse primer (max 35bp) 
9-mer approach 
Sequence (max 5000bp)
Leave it empty, if the primers are already modified